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[tigger623]

Hi,

I have been trying to isolate adult rat cardiomyocytes, but keep having issues with the cells - initially, I get rod-shaped cells, but after two hours, in which they are suppose to attach, the majority die and become round-shaped. They are beating when I initially look at them under the microscope, and I'm not sure if it's the beating that is causing them to die so quickly. I use a calcium-free buffer to perfuse, and then place them in different concentrations of calcium. Please help! I would be happy to send the protocol that I use. Thank you!