3 replies to this topic

[steinpdh]

Hi there,

I was wondering what gel percentage would be best to achieve the greatest separation between a protein of 24 kDa and GAPDH (36 kDa), since I subsequently wanted to cut the PVDF membrane in between. I don't have any beta-Actin antibodies right now.

[mdfenko]

you should get adequate separation with a 10 or 12% gel. test run with standards to ensure that you will get the resolution you require.

[memari]

you can use pre-cast gradiant gel.

http://www.piercenet...C7-14F7CD64CE52

I have used Precise Protein Gels, 4-20%, 10-Well Cat#25204.

They are easy to use and quick and perfect.

They sell this pre-cast gel in three different well capacity. 25ul, 30ul and 50ul.


Babak

[mdfenko]

memari, on 09 May 2012 - 08:57 AM, said:

you can use pre-cast gradiant gel.

http://www.piercenet...C7-14F7CD64CE52

I have used Precise Protein Gels, 4-20%, 10-Well Cat#25204.

They are easy to use and quick and perfect.

They sell this pre-cast gel in three different well capacity. 25ul, 30ul and 50ul.


Babak

should not use a gradient that long if you want to separate 24 and 36kDa with enough distance to cut apart from the membrane.

Edited by mdfenko, 09 May 2012 - 09:53 AM.