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Best gel percentage to separate 24 and 36 kDa proteins


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3 replies to this topic

#1 steinpdh

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Posted 09 May 2012 - 07:09 AM

Hi there,

I was wondering what gel percentage would be best to achieve the greatest separation between a protein of 24 kDa and GAPDH (36 kDa), since I subsequently wanted to cut the PVDF membrane in between. I don't have any beta-Actin antibodies right now.

#2 mdfenko

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Posted 09 May 2012 - 08:11 AM

you should get adequate separation with a 10 or 12% gel. test run with standards to ensure that you will get the resolution you require.
talent does what it can
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#3 memari

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Posted 09 May 2012 - 08:57 AM

you can use pre-cast gradiant gel.

http://www.piercenet...C7-14F7CD64CE52

I have used Precise Protein Gels, 4-20%, 10-Well Cat#25204.

They are easy to use and quick and perfect.

They sell this pre-cast gel in three different well capacity. 25ul, 30ul and 50ul.


Babak
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Babak Memari

#4 mdfenko

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Excellent

Posted 09 May 2012 - 09:52 AM

you can use pre-cast gradiant gel.

http://www.piercenet...C7-14F7CD64CE52

I have used Precise Protein Gels, 4-20%, 10-Well Cat#25204.

They are easy to use and quick and perfect.

They sell this pre-cast gel in three different well capacity. 25ul, 30ul and 50ul.


Babak

should not use a gradient that long if you want to separate 24 and 36kDa with enough distance to cut apart from the membrane.

Edited by mdfenko, 09 May 2012 - 09:53 AM.

talent does what it can
genius does what it must
i do what i get paid to do




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