2 replies to this topic

[nat2012]

Hi
I have recently started working with ThP1 cells with regard to production of IL-18 cytokines. I have seen many protocols saying the maturation of ThP1 is done by LPS or PMA.

I am a little confused with the whole set up but I assume I need to differentiate the cells using PMA and then stimulate them with LPS. Did I get that right?

What would you suggest is the best approach in my case?
Thanks

[zodiac1505]

I don't know much about this. But from what I have heard, THP-1 is a non adherent monocytic cell line. You usually stimulate them with PMA to enhance their maturation into macrophages and then they become adherent.

[chabraha]

Differentiate with PMA........anywhere from 50-200nM works...let them rest for 48-72hr post stimulus............then hit them with LPS.........you can check for activation via ELISA for TNF or IL6