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Using normal competent cells in electroporation method?


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#1 science noob

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Posted 08 May 2012 - 09:31 PM

Is it possible to electroporate normal competent cells (e.g. JM109) or electrocompetent cells are the only way to go?

#2 phage434

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Posted 09 May 2012 - 04:47 AM

You can easily make electrocompetent cells from normal (non-competent) cells. Just grow a culture to OD 0.6, wash twice with cold pure water, and concentrate about 200x (If you start with 200 ml, your final volume should be 1 ml). If you want to freeze them, do a final wash in 10% glycerol.
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