0
blunt end restriction out of recognition site
Started by snusnu, May 08 2012 10:55 AM
restriction blunt end cloning
No replies to this topic
#1
snusnu
-
- Members
-
- 2 posts
member
0
Neutral
Neutral
Posted 08 May 2012 - 10:55 AM
Hi all,
I'm looking for a restriction enzyme that will make blunt cleavage out of it's recognition site.
[color=#0066FF !important]For example:
if the recognition site is: CAAT
and the sequence is: CAGACAATGCT
GTCTGTTACGA
The resulting cleavage will be something like: CAGA....CAAT....GCT
GTCT....GTTA....CGA
Can any one [color=#0066FF !important]help me[/color] out?
Thank![/color]
Also tagged with one or more of these keywords: restriction, blunt end, cloning
 |
Protocols and Techniques Forums →
Molecular Cloning →
classic cloning with restriction enzymes into empty pcDNA3.1DStarted by Guest_Euterpa12_* , 17 May 2013  cloning, pcDNA3.1
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Cloning large fragmentsStarted by Guest_bknm_* , 15 May 2013 Cloning, PCR, large inserts
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Transformation colonies does not contain insertStarted by Guest_Sandy Fowler_* , 08 May 2013 transformation, Ligation, Cloning
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Need urgent advise..Started by Guest_M.T2013_* , 29 Apr 2013 cloning
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
|
|
|
