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blunt end restriction out of recognition site

restriction blunt end cloning

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#1 snusnu

snusnu

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Posted 08 May 2012 - 10:55 AM


Hi all,

I'm looking for a restriction enzyme that will make blunt cleavage out of it's recognition site.

[color=#0066FF !important]For example:

if the recognition site is: CAAT
and the sequence is: CAGACAATGCT
GTCTGTTACGA
The resulting cleavage will be something like: CAGA....CAAT....GCT
GTCT....GTTA....CGA

Can any one [color=#0066FF !important]help me[/color] out?
Thank!
[/color]





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