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an effective way to do a yeast colony pcr


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#1 zogene

zogene

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Posted 08 May 2012 - 05:26 AM

Hi bioforumers

Is any one know an effective way to do a yeast colony pcr because I have tried many but no results, so if you tried one and it worked fine with you can you provide me?

many thanks in advance

"Be the change that you wish to see in the world"


#2 pDNA

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Posted 08 May 2012 - 12:44 PM

happily i just realized that i have all the info availabe from an abstract :)
  • boiling in NaOH [1] or in cracking buffer,
  • freeze-thawing in lysis buffer [2]
  • or in the microwave oven [3],
  • SDS treatment [4],
  • heating in the microwave oven [5],
  • spheroplast preparation [6],
  • fast preparation of fungal DNA described by Liu et al. [7]
1. Wang, H., S.E. Kohalmi, and A.J. Cutler. Analytical Biochemistry, 1996. 237(1): p.
145-146.
2. Harju, S., H. Fedosyuk, and K.R. Peterson. Bmc Biotechnology, 2004. 4.
3. The Murray Lab from: http://www.mcb.harva...olony_pcr.html.
4. Akada, R., T. Murakane, and Y. Nishizawa. Biotechniques, 2000. 28(4): p. 668-+.
5. Lisby, M. from: www1.bio.ku.dk/english/research/fg/transkription/resources/protocols/colonypcr/.
6. Ling, M.F., F. Merante, and B.H. Robinson. Nucleic Acids Research, 1995. 23(23): p.
4924-4925.
7. Liu, K.H., Y.L. Yeh, and W.C. Shen. Journal of Microbiological Methods, 2011.
85(2): p. 170-172.

any questions? Posted Image

Regards,
p

#3 zogene

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Posted 09 May 2012 - 07:45 AM

Hello

thanks alot pDNA for response but I did not find the article link if you do have it would you give it to me, the point is I want a method that already somebody tried it already because there are many out there and I tried some and will try this one either.

many thanks again

"Be the change that you wish to see in the world"





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