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Fly cross help


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#1 mcb56

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Posted 07 May 2012 - 04:02 PM

Hello,

I would like to make a fly line that will express my protein of interest (MPI) through theGal4-UAS system. I have a fly line with the UAS-MPI and another fly line with Actin-Gal4 switch. I would like to make one line homozygous for UAS-MPI and Actin-Gal4 switch so I can induce expression of MPI at any point by feeding flies with RU486. I would also like to follow the crosses with a clear phenotype, not by doing PCR to follow them.

These are the genotypes of the 2 fly lines I have:

y[1] w[*]; P{w[+mC]=UAS-MPI.P}2 and

P{ry[+t7.2]=hsFLP}22, y[1] w[*]; P{w[+mC]=UAS-GFP.S65T}Myo31DF[T2]; P{w[+mC]=Act5C(-FRT)GAL4.Switch.PR}3/TM6B, Tb[1]

I can get other lines with various balancers to do crosses with if needed.

Thank you for any help you can provide.

#2 hobglobin

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Posted 08 May 2012 - 08:10 AM

But what is the question?
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#3 mcb56

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Posted 08 May 2012 - 08:51 AM

The question is how do I make the UAS-MPI; Actin-Gal4 switch fly?




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