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Conversion of ng/ul of DNA to ng for a PCR reaction

pcr concentration conversion ng

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#1 kel910

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Posted 07 May 2012 - 01:05 PM

So Im a little confused, I am doing a PCR reaction and require 200ng of genomic DNA template, the concentration of my genomic DNA is 34ug/ml (34ng/ul). So is 200ng of template the same as saying 200ng/ul? In that case I would just use C1V1=C2V2 to work out the volume of genomic DNA to use. Is this correct? I am using a 50ul reaction

#2 bob1

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Posted 07 May 2012 - 02:32 PM

NO! 200 ng is the total amount, use v=n/c

#3 Kamran

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Posted 09 May 2012 - 03:40 AM

add 5.88 ul template





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