So Im a little confused, I am doing a PCR reaction and require 200ng of genomic DNA template, the concentration of my genomic DNA is 34ug/ml (34ng/ul). So is 200ng of template the same as saying 200ng/ul? In that case I would just use C1V1=C2V2 to work out the volume of genomic DNA to use. Is this correct? I am using a 50ul reaction
Submit your paper to J Biol Methods today!
Conversion of ng/ul of DNA to ng for a PCR reaction
Started by kel910, May 07 2012 01:05 PM
pcr concentration conversion ng
2 replies to this topic
Also tagged with one or more of these keywords: pcr, concentration, conversion, ng
 |
Protocols and Techniques Forums →
General Lab Techniques →
Sonication of small PCR ampliconStarted by ForEpi, 22 Jul 2014  PCR, sonication
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
5' RLM RACEStarted by Epigeneticist, 09 Jul 2014 TOTAL RNA, PCR, RNA PURIFICATION and 1 more...
|
|
|
|
Protocols and Techniques Forums →
Molecular Biology →
Troubleshooting qPCR standardsStarted by janaki, 02 Jul 2014 qpcr, rtpcr, pcr, cloning
|
|
|
||
 Answered
Protocols and Techniques Forums →
Molecular Biology →
troubleshooting stubborn PCRStarted by Zduan, 01 Jul 2014 PCR, mol bio
|
|
|
||
Student Forums (for study, homework and non technical questions) →
-Molecular Biology- →
Conversion helpStarted by Mad researcher, 01 Jul 2014 Conversion, calculation and 1 more...
|
|
|
