So Im a little confused, I am doing a PCR reaction and require 200ng of genomic DNA template, the concentration of my genomic DNA is 34ug/ml (34ng/ul). So is 200ng of template the same as saying 200ng/ul? In that case I would just use C1V1=C2V2 to work out the volume of genomic DNA to use. Is this correct? I am using a 50ul reaction
Submit your paper to J Biol Methods today!
Conversion of ng/ul of DNA to ng for a PCR reaction
Started by kel910, May 07 2012 01:05 PM
pcr concentration conversion ng
2 replies to this topic
Also tagged with one or more of these keywords: pcr, concentration, conversion, ng
Protocols and Techniques Forums →
Molecular Biology →
Odd issue converting mutiplex PCR to single reactionsStarted by MealyWorm, 18 Aug 2017  PCR, multiplex PCR, Multiplexing and 2 more...
|
|
|
||
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
problem with PCR amplification of cDNAStarted by roshni mohan, 05 Aug 2017 PCR, Cloning, cDNA
|
|
|
||
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Problematic DNA ExtractionStarted by hmwmx, 14 Apr 2017 extraction, pcr, dna
|
|
|
||
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Recombinant DNA vaccine productionStarted by Mashudu, 02 Feb 2017 Primer design, Molecular biology and 3 more...
|
|
|
||
Protocols and Techniques Forums →
siRNA, microRNA and RNAi →
Where can I find 100-200 unique primer sequences for preparation of arrayStarted by Bio21, 16 Jan 2017 shRNA, array, PCR, primer
|
|
|
