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urgent help plz with RT-PCR

rt-pcr

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3 replies to this topic

#1 Bassam27

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Posted 07 May 2012 - 10:25 AM

Can any ONE have experience in RT PCR tell me how the measure the over expression of mRNA by using housekeeping gene ? do u measuring it by automated machine or you have to ues agrose gel. what is the best to do to measure the quantitative of mRNA?

#2 Curtis

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Posted 09 May 2012 - 07:08 PM

It is best to use Real-Time PCR machines for this kind of measurement.

#3 Bassam27

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Posted 25 July 2012 - 02:30 AM

when u uesing housekeeping gene , do i need to use sypree green dey or taqman? plz help me bcz i do not have any exp.

#4 Trof

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Posted 30 July 2012 - 07:28 AM

When you have no experience with real-time, best way is to first search and read something about it.
You can start with this forum topic - Important literature for real time PCR users

You can then ask about specific things you don't completely understand.

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