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Freezing down brain homogenate a good idea?


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#1 JacobThomas

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Posted 06 May 2012 - 08:31 PM

Hi, i'm wondering if freezing down a brain homogenate will have any detrimental effect to the ability of receptors to bind ligands?

I'd probably freeze over 1 minute using liquid nitrogen.

Thanks

#2 bob1

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Posted 07 May 2012 - 02:34 PM

Potentially, however snap-freezing (as you are doing) is probably the best way to preserve the proteins in a functional state if you need to store them




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