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HeLa migration

culture conditions migration

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#1 melitc

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Posted 06 May 2012 - 08:20 PM

Hi,

Does someone know if HeLa cells migrate under standar culture conditions (DMEM, 10% FBS, 37 C, 5% CO2), if yes, how can I to stop cell migration? if not how can I to induce cell migration? I would like to have two groups: migrating cells and not migrating cells for subsequently carry out patch clamp on those group of cells.

Thanks!

#2 bob1

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Posted 07 May 2012 - 02:46 PM

Yes they do. I don't know how to stop it, unless perhaps you can force the cells into G0 by some means, such as serum starvation.





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