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How to transfer His-tag from N- to C-terminus in plasmid


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#1 saad

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Posted 06 May 2012 - 06:04 PM

Hi Guys

I was wandering about one of my protein that I want to change the His-tag from N-terminal end to C-terminus with thrombine site. I don't know how to do it. Could some one help me which the easiest/quickest way to do it.

Thanks a lot

#2 bob1

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Posted 07 May 2012 - 02:51 PM

The easiest will be to subclone into a vector with a c-terminal tag, or to PCR out the gene using primers that include the His tag sequence.




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