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recover old transformed DH5

E.Colli transformed cells

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#1 claudia

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Posted 05 May 2012 - 11:35 AM

Does anyone knows how to recover transformed DH5 from an old plate kept at 4C?

#2 phage434

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Posted 05 May 2012 - 04:11 PM

If there are still viable cells, then restreak on a new plate. If not, you can miniprep the dead colonies and get small amounts of plasmid, which can be re-transformed.





Also tagged with one or more of these keywords: E.Colli, transformed cells

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