Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

how do i make non-ionized resin?


  • Please log in to reply
1 reply to this topic

#1 mimiW

mimiW

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 04 May 2012 - 09:56 AM

hi guys, i am trying to follow a new protocol, which requires the mix of non-ionized resin with DE 52 resins. is there a way to simply mix equal amount of anion and cation resins to form the non-ionized resin or i'll have to purchase from bio-rad????

Thanks for the help!!!!

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,704 posts
123
Excellent

Posted 07 May 2012 - 10:39 AM

i'm not sure what they mean, but you can buy plain cellulose packing material.

if you mix anion and cation exchangers they don't cancel each other out. you will end up with a mixed-bed ion exchanger. anions will bind to the anion exchanger and cations will bind to the cation exchanger and both will elute with a salt and/or pH gradient.

Edited by mdfenko, 07 May 2012 - 10:40 AM.

talent does what it can
genius does what it must
i do what i get paid to do




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.