Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

immunostaining of cells in culture


  • Please log in to reply
1 reply to this topic

#1 SF_HK

SF_HK

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 195 posts
2
Neutral

Posted 03 May 2012 - 09:46 PM

Hi,

I have to test for the presence of stem cells in my cell line by immunohistochemical staining (IHC). I've done IHC on formalin fixedparaffin embedded block but I don't know how I can fiz the cells in culture.

Any advice would be much appreciated.

Thank you

#2 Rsm

Rsm

    Post Dog

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 376 posts
8
Neutral

Posted 04 May 2012 - 06:03 AM

Culture your cells on cover slips. In the well: wash with PBS, fix with 4%PFA in PBS 5min @RT. Wash with PBS +0.1% Triton X100 2x5min. Block, stain, wash, stain, wash, mount.
I got soul, but I'm not a soldier




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.