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Help !! How to induce early apoptosis without using chemical agents in c

cell immunology cell biology apoptosis

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#1 CMIRC

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Posted 02 May 2012 - 12:34 PM

Hello

I am trying to induce early apoptosis in an adherent cancer cell line (PC3M), however whatever protocol I use I end up getting either 80% viability or a jump to late apoptosis. I would be grateful if somebody could suggest a method through which I can get early apoptosis. However I can not use chemicals to induce apoptosis. Please Help.

#2 bob1

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Posted 02 May 2012 - 06:12 PM

You can use UV (100 mJ) or gamma irradiation. For early apoptosis you wll need to start looking quite early - within a few hours of the treatment.

#3 CMIRC

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Posted 04 May 2012 - 05:09 AM

Thank you Bob 1 for the suggestion. I was thinking to do that.

However what do you think about heat induction, and regarding UV wouldn't the DNA of the cells be affected?

#4 bob1

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Posted 05 May 2012 - 02:27 PM

Never tried heat. With UV and gamma irradiation the DNA is damaged, this is typically what induces apoptosis!





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