- Anneal Linker 1(phospolilated 1a oligo + phospolilated 1b oligo)
Anneal Linker 2(phospolilated 2a oligo + phospolilated 2b oligo)
Ligated Linker 1+2 with T4, gel purify correct size
Digest and CIP vector
Ligate Vector with Linker (1+2)
Due to no digestion site and price(of oligo synthesis) I am looking for alternatives.
Thank you in advance for your help!!!!