i m working for expression of protein.my problem is that there is an extra chunk of protein .how can i separate it to get pure protein of my gene .
Submit your paper to J Biol Methods today!
How to get pure protein of a gene
1 reply to this topic
Posted 02 May 2012 - 08:01 AM
extra chunk? I assume you ran your purified protein on a gel and you are getting multiple bands. The best way to get the protein that you want from this mixture is size exclusion by FPLC. See if you have a column available and try that.