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[saimasbs]

i m working for  expression of protein.my problem is that there is an extra chunk of protein .how can i separate it to get pure  protein of my gene .

[HOYAJM]

extra chunk? I assume you ran your purified protein on a gel and you are getting multiple bands. The best way to get the protein that you want from this mixture is size exclusion by FPLC. See if you have a column available and try that.