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OVERDILUTION OF TBE BUFFER


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#1 helencollins560

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Posted 01 May 2012 - 03:11 PM

HI ALL

I wanted to make a 1x TBE solution from a 1 Litre bottle of Lonza Accugene 10x TBE buffer. However, instead of adding 9 Litres of milliq water to the buffer I added 10-11 litres of milliq water.

I wanted to know two things

[a] can i use this TBE as it is to make DNA gels for genotyping

or

[b] could i just get another bottle of 10x TBE add it to my existing stock then bring up to 20 litres with milliq to even out my error


thanks

#2 phage434

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Posted 01 May 2012 - 05:05 PM

It will probably work as is. You should make your gels with the same buffer.

You can fix this as you suggest by making additional buffer, if you would like.




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