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Homogenisation of protein

homogenisation polytron protein non-denaturing

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#1 JacobThomas

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Posted 30 April 2012 - 06:44 PM

Hi, sorry if this a pretty basic topic :D. I'm looking to homogenise brain/spinal cord protein, run a non-denaturing western blot, and probe with a labelled drug to look for specific receptors.

1: Can i homogenise to the point of free receptors?
2: What type of homogeniser should i use, glass, polytron or sonication?
3: Will sonication destroy tertiary protein structure and prevent the drug from binding the receptor?

Thanks, Jacob

#2 mdfenko

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Posted 01 May 2012 - 11:40 AM

we always homogenize brain and spinal cord with teflon/glass tissue grinder (and a drill press).

if you use a low clearance homogenizer and an appropriate medium then you should be able to free your receptors.

sonication may denature the proteins.

here are a couple of tissue grinders.
talent does what it can
genius does what it must
i do what i get paid to do





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