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Rapid western blot buffer ??


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2 replies to this topic

#1 ickypimp

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Posted 30 April 2012 - 07:48 AM

Hi

we recently bought a Bio rad trans blot turbo semi dry transfer system, when used with their proprietary reagents (precast gels and membrane transfer packs) you can transfer in 3 minutes...

with a home brew gel and the biorad transfer pack... 7 mins

with a home brew gel and my own nitrocellulose/transfer buffer (tris/glycene/methanol) .. 35 minutes...

the buffer has a unique smell, kinds like isopropanol and PVA glue...

does anybody have the inside line on its composition or can you recommend an alternative buffer recipe to improve the speed of our transfers ??

Thanks

#2 bob1

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Posted 30 April 2012 - 12:25 PM

Check out the MSDS for at least some of the components.

#3 hrx

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Posted 04 April 2013 - 08:26 AM

Has anyone tried shorter transfer times with a home brew gel and my own nitrocellulose/transfer buffer (tris/glycene/methanol) or different buffer systems?

Thanks




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