DNA in methylation analysisDNA isolation metho
Posted 27 April 2012 - 05:57 AM
I have a question for you, DNA isolation method can influence in the DNA methylation analysis?? For example if you use two different kits to isolate DNA, that could affect to the DNA methylation??
Posted 27 April 2012 - 06:07 AM
I would say no. Especially if you use commercially available kit. DNA is DNA.
The same goes for Bisulfite reaction (EpiTect or Zymo).
But that is just my opinion.
Posted 08 May 2012 - 07:02 PM
All comments and communication are my own personal ones, and are not tied to any of my affiliations.
Posted 22 May 2012 - 06:59 AM
The reply to your question will probably depend on what type of methylation analysis you are planning.
To give you an idea... we run a company that provides genome-wide methylation sequencing services and we have seen that there is a difference in the quality of the DNA for this specific application between different commercially available kits.
We always recommend our customers to use the easyDNA kit by Invitrogen.