Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Primer design for miRNA-qRT PCR

miRNA qRT-PCR Real-Time PCR miRNA primer qPCR primer design primer guidelines

  • Please log in to reply
No replies to this topic

#1 Neanderthal

Neanderthal

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 42 posts
1
Neutral

Posted 27 April 2012 - 12:52 AM

Hello everyone,


This is already posted on miRNA discussion group. But I am resposting it because of its relevance. Please bear with me.:)

I am interested in doing qRT-PCR to validate my miRNA microarray analysis. In order to do miRNA qRT-PCR,

1, How do I design primers for my selected miRNAs?

2. Should I take mature or stem loop structure?

3. Is there any software avalibale for designing miRNA qRT-PCR primers?

4. Do we need any special chemicals/reagents for miRNA qRT-PCR other than the standared reagents used with SYBR green chemistry for normal qRT-PCR (by normal I meant qRT-PCR for genes like p53, GAPDH etc)

5. What should I use as my housekeeping gene in case of miRNA-qRT PCR?

If someone can provide a guidline for desiging miRNA-qRT PCR primers, it will be of immense help.

Thanks a lot in advance.

K.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.