Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Primary cell line


  • Please log in to reply
2 replies to this topic

#1 zienpiggie

zienpiggie

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 139 posts
3
Neutral

Posted 26 April 2012 - 10:09 AM

Hi all,

I bought a cell line derived from normal cells/nonmalignant cells from atcc, and it's been suggested in the literature that the cells are primary cells. I however met someone who argued that it's not really a primary cells because 1. it is not derived directly from the donor and the person said that primary cells would usually last only for one passage or so and that's it. 2. they are transformed because I can passage them up to 8 passages.

Also, some suggests that primary cells should never be freeze thawed upon arrival and be used within five passages?

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,505 posts
370
Excellent

Posted 26 April 2012 - 01:29 PM

Many primary cells (they aren't lines until transformed for more or less immortal growth) will grow well beyond the first couple of passages, many even up to passage 10 or more without being transformed. Transformation is the conversion to unregulated growth!

I suggest you have a look at a good cell culture manual (e.g. R.I Freshney, culture of animal cells) for more information.

#3 zienpiggie

zienpiggie

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 139 posts
3
Neutral

Posted 26 April 2012 - 04:15 PM

Thanks Bob1. I probably should pick up Freshney to refresh. Thanks for the confirmation!!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.