multiple bands for genomic DNA extractionbacterial dna extra
Posted 26 April 2012 - 05:34 AM
I have doing my final project about extracting genomic DNA from bacteria for PCR and RFLP.
I used this protocol for genomic dNA extraction:
1)cell lysis by SDS
2)Frreze and thaw method 3 times by liquid nitrogen
3)phenol chloroform extraction
but when the DNA was run on gel, it gave multiple bands instead of one band.
Could you please help me?
Posted 26 April 2012 - 11:41 AM
A few colleagues of mine, along with myself have been trying out newer methods for extraction of gDNA and we've come across a kit that is pretty interesting. It is called PureLyse by this company out of California called Claremont Bio. The protocol is very short and thus far I've had no problems adding my sample into PCR and getting proper amplification. Here's the link if you want to see it. http://www.claremont...reLyse_s/57.htm
Let me know if this helped ya out.
Posted 26 April 2012 - 01:02 PM