Getting genomic DNA for PCR
Posted 25 April 2012 - 11:24 PM
I wish to know get some genomic DNA (of any kind) for my experiment testing. I happen to saw a JM109 competent cell tube on the freezer. I wonder can I just use that one and run as PCR template?
Posted 26 April 2012 - 12:36 AM
Posted 26 April 2012 - 05:38 PM
If they are in broth, take as much as a colony and put it inside of PCR tube and mix it with a tip.
It always works.
I have done this more than 50 times without any problem.
We used to call it---> Colony-PCR
Edited by memari, 26 April 2012 - 05:40 PM.
Posted 26 April 2012 - 07:52 PM
Because I'm not doing cloning (not to mention that I do not have cloning equipment as well), I can't grow them and pick it. I only have the stock amount of competent cells stored in freezer.
Is it possible to take out a few ul of competent cells from the stock and then boils it and spins down for the gDNA as template?
Posted 26 April 2012 - 08:04 PM