identification by 16s rRNA
Posted 25 April 2012 - 07:06 AM
Posted 25 April 2012 - 07:43 AM
But by not matching: what do you mean? Its possible that it "doesnt", but that you are working with "family" (closely related) or that you get a real big difference... I mean a "real" non matching problem...
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.
Posted 25 April 2012 - 08:41 AM
Posted 25 April 2012 - 12:18 PM
Posted 25 April 2012 - 11:45 PM
However your problem can easily be solved by doing the "archaic" tests suggested by Phil especially gram staining - and they will include LOOKING at your bacteria - and microscopy will also (if you are not extremly unlucky) enable you to see a difference in the morphology of the cells (rods in case of pseudomonas, cocci for staph .....) which helps you to decide which results to trust more the DNA or the biochemistry. This will also show if you are working with a pure or mixed culture of all three - which might be the reason for your inconsistent results.