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'Standard salt buffer' for protein storage/biophysical techniques

proteins buffers phosphate

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#1 piotras

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Posted 25 April 2012 - 05:45 AM

Hi,

I'm beginning my 'adventure' with life sciences and I'm rather confused on what people consider to be the 'standard protein buffers'. I would like to assume average needs - fully understand that different proteins and techniques require sample-specific components.

For example, I've got a 9.5kDa protein domain in a 20mM sodium phosphate buffer (ph 8) which I would like to use for some biophysical analysis. Can I assume that it currently has sufficient 'standard' folding conditions?

Thanks

#2 bob1

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Posted 25 April 2012 - 04:52 PM

Normal conditions for most (eukaryotic) proteins are conditions that are found in the body - Salt 0.9% (approx 150 mM NaCl for correct osmolarity), buffered to pH 7-8.





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