Hello everybody !
I have a question, regarding the Hoechst staining. There is 3 different Hoechst : 33580, 33258 and 33342. I know that 33258 and 33342 are very similar, excepted that 33258 is less cell-permeant (around 10 times).
The problem is that my microscope filters are better for Hoechst 34580, but it's the stain which is the less used. Why ? Is there a reason to it ?
Can you help me about the protocol to follow for a good staining ? (optimal concentration, for using and stock solutions...)
Thank you very much !
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