Dear BioForum members,
I am coating coverslips with poly-l-lysine followed by fibronectin to grow some primary neurons from chick. I have come across several protocols that direct me not to allow the poly-l-lysine to dry, and several that direct me to dry it after washing. What's the reasoning behind this?
I know fibronectin shouldn't be allowed to dry. Any thoughts on this matter would be much appreciated.
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