Dear BioForum members,
I am coating coverslips with poly-l-lysine followed by fibronectin to grow some primary neurons from chick. I have come across several protocols that direct me not to allow the poly-l-lysine to dry, and several that direct me to dry it after washing. What's the reasoning behind this?
I know fibronectin shouldn't be allowed to dry. Any thoughts on this matter would be much appreciated.
Thanks.
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Poly-l-lysine treated coverslips: To Dry or Not to Dry?
Started by hogthehedge, Apr 24 2012 11:05 PM
poly-l-lysine coverslips
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Also tagged with one or more of these keywords: poly-l-lysine, coverslips
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How do you coat coverslips before seeding cells?Started by Guest_jennycharlies_* , 18 Aug 2011  poly-d-lysine, cells, coverslips
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