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Does this qualify as a whole cell-based assay?

bioluminescence BRET whole cell assay

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#1 tdcmmon

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Posted 24 April 2012 - 04:48 PM

I'm optimizing an assay which is use a bioluminescent protein to detect changes in DAG levels in response to exposing cells to agonists.

The assay is conducting on a monolayer of cells in a 96-well plate and we're measuring the bioluminescence emitted from the cells after exposure to certain drugs.

Could this be, in anyway, considered a whole cell-based assay? It's obviously cell-based, but I figure since we cannot detect sub-cellular localization of the BRET signal (bioluminescence), it might qualify as whole cell.

#2 bob1

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Posted 25 April 2012 - 04:43 PM

Sure, if you are using the whole cell, it would be a whole cell assay.





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