Warning: mb_substr_count() expects at most 3 parameters, 4 given in /home/protocol/public_html/forums/admin/sources/classes/text/parser/bbcode/defaults.php on line 1269

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser/bbcode/defaults.php:1269) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 110

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser/bbcode/defaults.php:1269) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 127

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser/bbcode/defaults.php:1269) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 136

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser/bbcode/defaults.php:1269) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 137

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser/bbcode/defaults.php:1269) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 141
Phospholipase C activity measurement - Biochemistry - BioForum

Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Phospholipase C activity measurement


  • Please log in to reply
1 reply to this topic

#1 philman

philman

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 104 posts
5
Neutral

Posted 23 April 2012 - 03:06 AM

Hi,

I'm not sure which section to post this in entirely but Biochemistry seems to be the most logical.

I want to try and measure the activity of Phospholipase C in human endothelial cells and was wondering if anyone had any reliable techniques for measuring it.

I have looked at several papers online and the methods I have found appear to fall into immunoprecipitation followed by phospho-tyrosine immunoblotting, or measurement of enzyme activity with PIP3 or something. Does anyone know which method is more reliable or easier to reproduce?

I was also wondering if it was possible to do colocalisation studies under confocal microscopy.

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,182 posts
193
Excellent

Posted 23 April 2012 - 06:56 AM

worthington has a titrimetric assay procedure at this webpage: phospholipase c assay.

some people where i work assay(ed) with pip3 but i don't know the full procedure (it may be radiolabelled, i'm not sure, it's been a long time since i discussed this with them).
talent does what it can
genius does what it must
i do what i get paid to do




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.