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Check cell viablity after FACS

cell viability FACS live/dead staining

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2 replies to this topic

#1 RLI12

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Posted 23 April 2012 - 01:32 AM

Hi all,

I have some GFP positive cells that I sort by FACS. However, they don't look very happy when I put them in culture after the sorting and I think most of them are not surviving.

Could anyone recomend me any live/dead cell assays to check the viability after the sorting? Can I do this by adding PI during the sorting?

Many thanks in advance for the information!

#2 bob1

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Posted 26 April 2012 - 01:33 PM

Take an aliquot and add some trypan blue...live dead count on haemocytometer.

#3 angelafuertes

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Posted 07 April 2013 - 06:20 AM

You can add PI to selectively stain dead cells and even gate them out when you analyze your FACS data - have a look here. As bob1 was saying, you can also count live (unstained) and dead (stained) cells with a hemocytometer and get the viability from there.





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