Hi all,
I have some GFP positive cells that I sort by FACS. However, they don't look very happy when I put them in culture after the sorting and I think most of them are not surviving.
Could anyone recomend me any live/dead cell assays to check the viability after the sorting? Can I do this by adding PI during the sorting?
Many thanks in advance for the information!
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Check cell viablity after FACS
Started by RLI12, Apr 23 2012 01:32 AM
cell viability FACS live/dead staining
2 replies to this topic
#2
bob1
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Thelymitra pulchella
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Posted 26 April 2012 - 01:33 PM
Take an aliquot and add some trypan blue...live dead count on haemocytometer.
#3
angelafuertes
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Posted 07 April 2013 - 06:20 AM
You can add PI to selectively stain dead cells and even gate them out when you analyze your FACS data - have a look here. As bob1 was saying, you can also count live (unstained) and dead (stained) cells with a hemocytometer and get the viability from there.
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