Problems with Clostridium
Posted 22 April 2012 - 10:43 PM
I´m trying to grow Clostridium sphenoides in a specific liquid medium (Reinforced Clostridial medium) and also in Petri dishes that I have put inside an anaerobic jar. It seems that the bacteria grows in the liquid medium as it becomes muddy or cloudy. However, there is no growth in the agar plate. The medium I have used is adecuate,so I don´t know which the problem is. I would like to extract DNA from this bacteria so that´s why I prefer to inoculate it in an agar plate. Could anyone give me any idea or any advice about it? I would really appreciate it..
Posted 23 April 2012 - 08:10 AM
Posted 23 April 2012 - 08:11 AM
The liquid medium is usually a little hazy - are you sure you saw actual growth?
Posted 23 April 2012 - 11:48 PM
Posted 24 April 2012 - 12:27 AM
The aerobic one not growing in the plates does not sound normal...
Yes, I have used the same medium adding the 15g/l of agar, and you´re right maybe the storage of plates before inoculation could be a possible cause (I have used O2 indicator), what can I do in that case? I have the same problem with an aerobic bacteria and a facultative anaerobic one, they grow in liquid medium but not in the agar plates....
I am starting to think you are doing something "weird" (wrong) with your plates then...
Are you sure that the only difference between the plates and the liquid is the agar? And what about growht conditions? Temp? etc?
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.
Posted 24 April 2012 - 12:33 AM
Anyway thank you very much
Posted 24 April 2012 - 04:00 AM