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pH rise upon filtration


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#1 newbie99

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Posted 20 April 2012 - 05:04 PM

"The pH may rise 0.1 to 0.3 units upon filtration" (This is found in many Tissue Culture publications.)

Example:
pH is adjusted to 7.00. After filter sterilization, the pH becomes 7.10-7.30.

My questions:
Why does the pH rise upon filtration? What's the mechanism behind it?
Does the pH rise only in medium containing sodium bicarbonate? Does it also rise in PBS (Na-Bicarb free)?

Any thoughts are welcome.

#2 bob1

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Posted 22 April 2012 - 12:39 PM

Some of it probably depends on the filter type, but I suspect that the majority will be because of absorption of CO2 from the air during the collection part.

#3 mdfenko

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Posted 23 April 2012 - 07:03 AM

absorbing co2 would lower the pH.

sterile filtration will also somewhat degas the solution. the removal of co2 may, therefore, be the cause of rising pH.
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