pH rise upon filtration
Posted 20 April 2012 - 05:04 PM
pH is adjusted to 7.00. After filter sterilization, the pH becomes 7.10-7.30.
Why does the pH rise upon filtration? What's the mechanism behind it?
Does the pH rise only in medium containing sodium bicarbonate? Does it also rise in PBS (Na-Bicarb free)?
Any thoughts are welcome.
Posted 22 April 2012 - 12:39 PM
Posted 23 April 2012 - 07:03 AM
sterile filtration will also somewhat degas the solution. the removal of co2 may, therefore, be the cause of rising pH.
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