I am looking to enrich serum IgM for specific antigen utilizing weak affinity chromatography (wac). Protocols that I have encountered for WAC utilize HPLC, which I do not have access to. Is it possible to do this by FPLC or gravity with similar resolution?Any advice for enriching specific serum IgM with incredibly weak affinity in a gentle (nondenaturing) approach?
0
Weak affinity chromatography
Started by rjp, Apr 20 2012 07:04 AM
affinity chromatography IgM HPLC FPLC
2 replies to this topic
#2
Inmost sun
-
- Active Members
-
- 286 posts
Veteran
59
Excellent
Excellent
Posted 15 May 2012 - 02:41 AM
weak can mean "less specific"; you may use hydrophobic interaction chromatography where you can gear binding by salt concentration or pH...
#3
mdfenko
-
- Active Members
-
- 2,241 posts
an elder
78
Excellent
Excellent
Posted 15 May 2012 - 11:52 AM
if you can find the same packing phase as the hplc method then you can run fplc or/and conventional lc. fplc resolution should be similar, conventional may exhibit more band spreading due to slower elution.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
Also tagged with one or more of these keywords: affinity, chromatography, IgM, HPLC, FPLC
 |
Protocols and Techniques Forums →
Protein Expression and Purification →
Is it possible to use wireless connection to control and monitor FPLC?Started by Guest_mblouis_* , 08 May 2013  FPLC, wireless, wifi, AKTA
|
|
|
|
|
|
Protocols and Techniques Forums →
Protein and Proteomics →
Peptide HPLC Sample PrepStarted by Guest_Kb Homes_* , 22 Apr 2013 Peptide, purification, HPLC
|
|
|
|
Protocols and Techniques Forums →
Biochemistry →
Arginine on anione IECStarted by Guest_EvilTwin_* , 07 Nov 2012 chromatography, arginine and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Protein and Proteomics →
rpHPLC chromatograph shiftStarted by Guest_ionchannelbk_* , 23 Oct 2012 HPLC, protein separation
|
|
|
||
Protocols and Techniques Forums →
Biochemistry →
Lost of ReproducibilityStarted by Guest_Zeenat Tinwala_* , 09 Sep 2012 PGA, LC/MS, HPLC
|
|
|
