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Weak affinity chromatography

affinity chromatography IgM HPLC FPLC

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#1 rjp

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Posted 20 April 2012 - 07:04 AM

I am looking to enrich serum IgM for specific antigen utilizing weak affinity chromatography (wac). Protocols that I have encountered for WAC utilize HPLC, which I do not have access to. Is it possible to do this by FPLC or gravity with similar resolution?Any advice for enriching specific serum IgM with incredibly weak affinity in a gentle (nondenaturing) approach?

#2 Inmost sun

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Posted 15 May 2012 - 02:41 AM

weak can mean "less specific"; you may use hydrophobic interaction chromatography where you can gear binding by salt concentration or pH...

#3 mdfenko

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Posted 15 May 2012 - 11:52 AM

if you can find the same packing phase as the hplc method then you can run fplc or/and conventional lc. fplc resolution should be similar, conventional may exhibit more band spreading due to slower elution.
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