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Freezing bacteria


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4 replies to this topic

#1 vanu

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Posted 19 April 2012 - 02:49 AM

Hi,

I would like to know if it´s correct to freeze bacteria at -140 degrees with 20% of glycerol.

Thanks,

vanu

#2 pito

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Posted 19 April 2012 - 02:58 AM

yes, that is possible
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.

#3 vanu

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Posted 19 April 2012 - 05:28 AM

Really? They won´t suffer any harm, because everywhere I´ve seen that they should be frozen at -80degrees ....

Thank you very much,

#4 pito

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Posted 19 April 2012 - 05:51 AM

Really? They won´t suffer any harm, because everywhere I´ve seen that they should be frozen at -80degrees ....

Thank you very much,


Check this: http://www.cabri.org.../M300Ap501.html

A lot of labs just use -80°C because they have the equipment for it.
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.

#5 vanu

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Posted 19 April 2012 - 08:14 AM

Thanks, that helps.




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