4 replies to this topic

[vanu]

Hi,

I would like to know if it´s correct to freeze bacteria at -140 degrees with 20% of glycerol.

Thanks,

vanu

[pito]

yes, that is possible

[vanu]

Really? They won´t suffer any harm, because everywhere I´ve seen that they should be frozen at -80degrees ....

Thank you very much,

[pito]

vanu, on 19 April 2012 - 05:28 AM, said:

Really? They won´t suffer any harm, because everywhere I´ve seen that they should be frozen at -80degrees ....

Thank you very much,

Check this: http://www.cabri.org.../M300Ap501.html

A lot of labs just use -80°C because they have the equipment for it.

[vanu]

Thanks, that helps.