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NL and L?


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#1 new learner

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Posted 19 April 2012 - 01:40 AM

Does anyone know what is the differences between non linear and linear IPG strips???From my reading many of the researchers use NL IPG strips....why????

#2 proteaMatt

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Posted 23 April 2012 - 11:40 AM

Linear strips have a linear pH gradient, meaning it transitions from high pH to low pH uniformly throughout the strip.
Non-linear strips have a non-linear pH gradient, meaning the transition from high pH from low pH is not uniform throughout the strip. For example if you are using a 3-7 NL 11cm strip, the gradient might change from 3-5 over the first 4cm and then 5-7 over the next 7 cm, this would help you to separate proteins with similar iso-electric points around pH 7.
Lab Technician at Protea Biosciences

#3 new learner

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Posted 26 April 2012 - 07:32 PM

Thanks proteaMatt~ Posted Image




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