I am looking to enrich serum IgM for specific antigen utilizing weak affinity chromatography (wac). Protocols that I have encountered for WAC utilize HPLC, which I do not have access to. Is it possible to do this by FPLC or gravity with similar resolution?Any advice for enriching specific serum IgM with incredibly weak affinity in a gentle (nondenaturing) approach?
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Weak Affinity Chromatography
Started by rjp, Apr 18 2012 05:55 AM
chromatography biochemistry fplc hplc
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Also tagged with one or more of these keywords: chromatography, biochemistry, fplc, hplc
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