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Weak Affinity Chromatography

chromatography biochemistry fplc hplc

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#1 rjp



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Posted 18 April 2012 - 05:55 AM

I am looking to enrich serum IgM for specific antigen utilizing weak affinity chromatography (wac). Protocols that I have encountered for WAC utilize HPLC, which I do not have access to. Is it possible to do this by FPLC or gravity with similar resolution?Any advice for enriching specific serum IgM with incredibly weak affinity in a gentle (nondenaturing) approach?

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