Posted 17 April 2012 - 10:07 AM
I have plasmid with my insert around 6700bp. And after double digestion, I m expect ion bands 400bp and 6300p but in the gel I got the bands around 8000bp.
Anyone can explain what will be reason ?
Posted 17 April 2012 - 10:23 AM
Your insert is 6700bp.. but how big is your vector?
ANd you get 1 band of 8000 bp?
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.
Posted 21 April 2012 - 04:13 AM
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon