Posted 17 April 2012 - 10:07 AM
I have plasmid with my insert around 6700bp. And after double digestion, I m expect ion bands 400bp and 6300p but in the gel I got the bands around 8000bp.
Anyone can explain what will be reason ?
Posted 17 April 2012 - 10:23 AM
Your insert is 6700bp.. but how big is your vector?
ANd you get 1 band of 8000 bp?
Posted 21 April 2012 - 04:13 AM
I never trust anything that can't be doubted.