Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Digestion

Fermentas enzyme

  • Please log in to reply
2 replies to this topic

#1 mayja85

mayja85

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 17 April 2012 - 10:07 AM

Hi all,

I have plasmid with my insert around 6700bp. And after double digestion, I m expect ion bands 400bp and 6300p but in the gel I got the bands around 8000bp.

Anyone can explain what will be reason ?

#2 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,334 posts
81
Excellent

Posted 17 April 2012 - 10:23 AM

Hard to tell based on what you explain here.

Your insert is 6700bp.. but how big is your vector?
ANd you get 1 band of 8000 bp?

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#3 Trof

Trof

    Brain on a stick

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,201 posts
109
Excellent

Posted 21 April 2012 - 04:13 AM

How did your uncut plasmid look on the gel?

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.