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Lifespan of HRP-conjugated Secondary antibody @ Room temperature?

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2 replies to this topic

#1 science noob

science noob


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Posted 16 April 2012 - 08:02 PM

I've accidentally left a secondary HRP antibody stock thawing out on ice overnight from -20C. Forgot about it and when I remembered I had something in that ice box, all the ice has melted and the vial is floating in melted water. Temperature of that water is estimated to be 10 to 20C.

Should I just discard the whole vial assuming everything has been degraded? How long can antibodies stay at room temperature until it goes off? Can I still use it (decreases binding etc?)?

#2 BioMiha



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Posted 16 April 2012 - 10:09 PM

Antibodies normally work at 37 °C so in all logic they should be fine. I usually don't freeze my antibody aliquotes and they work with no problems for a long time.

Edited by BioMiha, 16 April 2012 - 10:10 PM.

#3 Sotirios



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Posted 17 April 2012 - 01:05 PM

The antibody is ok. If there will be a problem, it would be with enzyme (HRP). Anyway, I have done many stress test with these and dont worry.
Athanasiou Sotirios

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