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Fluorescence through Plastic

fluorescence plastic

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2 replies to this topic

#1 azileretsis

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Posted 16 April 2012 - 01:38 PM

Hi,

I'm selecting virus plaques based on GFP on an inverted microscope. Has anyone noticed a dampening effect of GFP based on type of plasticware, 35mm dishes vs. 6-well plates? Is there a good cell culturing plasticware that does not hinder GFP fluorescence?

#2 Curtis

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Posted 16 April 2012 - 10:38 PM

Do you have any controls? I don't think the plastic is the issue. Maybe a virus plaque doesn't glow like a transfected cell.

#3 leelee

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Posted 17 April 2012 - 05:21 AM

I do this frequently (i.e. grow viruses in tc expressing GFP) and I haven't experienced any dampening by plastic. I've used 96 well, 6 well trays and T75 flasks.





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