2 replies to this topic

[Chelo]

Dear all,

is it possible to use the classical solution D followed by phenol/chloroform to extract RNA?
I was told that going directly to phenol or Trizol gives lower yields. Since the solution D contains a detergent, I thought it could be a better for the purification.
Thanks in advance!

[96well]

You may want to check the instructions from the Urine RNA extraction kit, maybe it is required to add some buffer similar to your solution D.

[romaoleao]

It is possible to do RNA extraction in urine samples after its fresh frozen in liquid nitrogen and storage at -80ºC?  It is necessary to have any preservation solution as RNA later or other to preserve urine??? Thanks