I am trying to clone a gene on an inducible vector using gateway system. The sequence seems to be correct. however, when I transfect HEK293s with my clone, there is no overexpression of the protein. The way I do is to transfect using lipo 2000, induce the day after and harvest 24h post induction.
Any ideas and help is highly appreciated
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Cloning into inducible vector but no expression
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