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Cloning into inducible vector but no expression


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#1 Ambinlab

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Posted 16 April 2012 - 07:48 AM

Hi All,

I am trying to clone a gene on an inducible vector using gateway system. The sequence seems to be correct. however, when I transfect HEK293s with my clone, there is no overexpression of the protein. The way I do is to transfect using lipo 2000, induce the day after and harvest 24h post induction.

Any ideas and help is highly appreciated

#2 zodiac1505

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Posted 18 October 2012 - 08:47 AM

Are you sure you are giving it enough time?




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