Hello all,
I have some plant root tissues that I would like to store at -80 for future RNA extractions. I was wondering if It was ok to store the tissue at -80, then quickly transfer it to a homogenization tube prior to extraction? This would probably unthaw the tissue somewhat. Would this pose a risk to my RNA?
Thanks in advance!
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Storage of tissue at -80 followed by a breif unthaw prior to extraction
Started by tecvin, Apr 16 2012 07:35 AM
RNA extraction tissue
1 reply to this topic
#2
bob1
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Thelymitra pulchella
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Excellent
Excellent
Posted 16 April 2012 - 05:49 PM
Potentially it may cause some degradation. If you have access to dry ice or LN2 you could try grinding the tissue in the frozen state, which should prevent this problem.
