Hello all,
I have some plant root tissues that I would like to store at -80 for future RNA extractions. I was wondering if It was ok to store the tissue at -80, then quickly transfer it to a homogenization tube prior to extraction? This would probably unthaw the tissue somewhat. Would this pose a risk to my RNA?
Thanks in advance!
Submit your paper to J Biol Methods today!
Storage of tissue at -80 followed by a breif unthaw prior to extraction
Started by tecvin, Apr 16 2012 07:35 AM
RNA extraction tissue
1 reply to this topic
#2
bob1
-
- Global Moderators
-
- 4,877 posts
Thelymitra pulchella
291
Excellent
Excellent
Posted 16 April 2012 - 05:49 PM
Potentially it may cause some degradation. If you have access to dry ice or LN2 you could try grinding the tissue in the frozen state, which should prevent this problem.
Also tagged with one or more of these keywords: RNA, extraction, tissue
 |
Protocols and Techniques Forums →
Genetics and Genomics →
question about RNA concentration for real time PCRStarted by clwaldru, 02 Dec 2013  PCR, real time PCR, RNA, nanodrop
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Biology →
rna isolation from formalin fixed peg tissueStarted by mdfenko, 14 Nov 2013 rna, formalin fixed, peg
|
|
|
|
|
|
Protocols and Techniques Forums →
General Lab Techniques →
Megaplasmid best extraction protocolStarted by alanlucid, 07 Nov 2013 plasmid, extraction, megaplasmid
|
|
|
|
Protocols and Techniques Forums →
Molecular Biology →
DNA contamination from adenovirus afted DNase treatmentStarted by juelraja, 26 Sep 2013 RNA, adenovirus, extraction and 2 more...
|
|
|
||
 Answered
Protocols and Techniques Forums →
Molecular Biology →
How to ship plant tissue for RNA extraction?Started by aodonnell311, 21 Sep 2013 RNA, RNA extraction, plant tissue and 2 more...
|
|
|
