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Storage of tissue at -80 followed by a breif unthaw prior to extraction

RNA extraction tissue

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#1 tecvin

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Posted 16 April 2012 - 07:35 AM

Hello all,

I have some plant root tissues that I would like to store at -80 for future RNA extractions. I was wondering if It was ok to store the tissue at -80, then quickly transfer it to a homogenization tube prior to extraction? This would probably unthaw the tissue somewhat. Would this pose a risk to my RNA?

Thanks in advance!

#2 bob1

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Posted 16 April 2012 - 05:49 PM

Potentially it may cause some degradation. If you have access to dry ice or LN2 you could try grinding the tissue in the frozen state, which should prevent this problem.





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