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Problem with polyacrylamide gel when electrophoresis in long time

streak DNA electrophoresis SSCP

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#1 binhnguyen1000

binhnguyen1000

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Posted 15 April 2012 - 04:30 AM

Hi !
I use protein II xi cell system to electrophoresis DNA (for SSCP analysis), this is some infomation in my protocol:
PAGE 12% nonedenaturing, bis:acrylamide=1:49
20*16cm gel
TAE1X
4 degrees celcius
Electrophoresis in 12h
But i have problem with gel after silver staining, see this image:
Posted Image
this streack go down bottom gel during electrophoresis and breck band DNA.
what is problem? please help!





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