Amplification curves jagged
Posted 13 April 2012 - 11:10 AM
- boko likes this
Posted 13 April 2012 - 01:46 PM
That happens. No more than two runs of one plate, and even the second can be a little bit zig-zagged.
Try the High specifity setting in the 2nd Derivative analysis module, it should make correct Cts.
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