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How to test for the existence of a mutated microRNA?

microRNA Dicer Drosophila stem-loop

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#1 kaveh



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Posted 13 April 2012 - 08:48 AM


I do in-vivo gain-of-function studies with Drosophila microRNAs. I have mutated the seed sequence of a particular microRNA gene and have made transgenic flies that express that mutated transcript. Of course, the transcript doesn't exactly make the same stem-loop structure as its parent. Therefore, the Dicer-1 cutting site might be different or none!

Can RT-PCR (with specific primers) tell me if that putative mutated microRNA exist?

Any input is highly appreciated!


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