Hello,
I do in-vivo gain-of-function studies with Drosophila microRNAs. I have mutated the seed sequence of a particular microRNA gene and have made transgenic flies that express that mutated transcript. Of course, the transcript doesn't exactly make the same stem-loop structure as its parent. Therefore, the Dicer-1 cutting site might be different or none!
Can RT-PCR (with specific primers) tell me if that putative mutated microRNA exist?
Any input is highly appreciated!
Kaveh
0
How to test for the existence of a mutated microRNA?
Started by kaveh, Apr 13 2012 08:48 AM
microRNA Dicer Drosophila stem-loop
No replies to this topic
Also tagged with one or more of these keywords: microRNA, Dicer, Drosophila, stem-loop
 |
Protocols and Techniques Forums →
siRNA, microRNA and RNAi →
how to platelet purity with dynabeadsStarted by Guest_bensonvicky_* , 22 Apr 2013  platelet purity, microRNA and 1 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
siRNA, microRNA and RNAi →
Overexpressing miR - rescue by co-overexpressing its targetStarted by Guest_PBT_* , 31 Mar 2013 miRNA, microRNA, overexpression and 2 more...
|
|
|
|
Protocols and Techniques Forums →
Molecular Biology →
Staining lysed cells in DrosophilaStarted by Guest_levuccio_* , 14 Feb 2013 lysed, drosophila, phage, virus
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Culture of Drosophila S2R+ cellsStarted by Guest_Dai Le_* , 29 Dec 2012 S2R+, culture, drosophila
|
|
|
||
Protocols and Techniques Forums →
siRNA, microRNA and RNAi →
microRNA promoter based expression vector?Started by Guest_assembler01_* , 07 Dec 2012 microRNA, expression
|
|
|
