I have labeled cells with 3.16 uM CFSE for 5 minutes, washed, and setup cells for culture (stimulated cultures using 1 ug/ml of SEB). After analysis by FACS, I had expected to see several peaks on the histogram of CFSE after 3 days of culture indicating proliferation. However, there is only a single peak ?
Why dont I get several nice peaks showing each cell division ?
If anyone has got experience using CFSE on human PBMC please comment.
CFSE labeling of human PBMC
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