5 replies to this topic

[Benchworker]

Hi,

I was heard that when the confluence of cultured H9c2 cells was over 70%. Cells would lose their own properties. Can anybody explain the detailed underlying mechanism?

Thanks!

[bob1]

This sounds suspiciously like homework, so I'm not going to give a full answer:

When you culture many cell types, but especially primary ones, what happens to the cells as the cells approach confluence?  How would this effect change RNA and protein levels in the cells?

[Benchworker]

bob1, on 12 April 2012 - 01:20 PM, said:

This sounds suspiciously like homework, so I'm not going to give a full answer:

When you culture many cell types, but especially primary ones, what happens to the cells as the cells approach confluence?  How would this effect change RNA and protein levels in the cells?

It's not a homework, seriously! And I am really want to know the answer. Thanks!

[bob1]

Have you heard of contact inhibition or contact senescence?

Edited by bob1, 17 April 2012 - 02:33 PM.

[Benchworker]

bob1, on 17 April 2012 - 02:33 PM, said:

Have you heard of contact inhibition or contact senescence?

Actually, H9c2 is not a kind of primary cells. Does confluence density have same effect on the cell lines????

[bob1]

Many of them, yes.  This is why you should subculture your cells before they become too confluent.